Coding

Part:BBa_K1973016:Design

Designed by: Laura Claret Fernndez   Group: iGEM16_UPO-Sevilla   (2016-10-09)


pleD*


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 29
    Illegal BamHI site found at 1186
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 444
    Illegal NgoMIV site found at 591
    Illegal AgeI site found at 927
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 102


Design Notes

We have included the sequence of RBS BBa_B0034 by means of the PCR primers and amplification, as well as the scar that should have been in the clonning of this part.


Source

It was obtained form a plasmid that contained its sequence.

References